noise2read Documentation

noise2read, originated in a computable rule translated from PCR erring mechanism that: a rare read is erroneous if it has a neighboring read of high abundance, turns erroneous reads into their original state without bringing up any non-existing sequences into the short read set(<300bp) including DNA and RNA sequencing (DNA/RNA-seq), small RNA, unique molecular identifiers (UMI) and amplicon sequencing data.

Note

The easy-usable and automatic tuning of the classifiers’ parameters facilitates wide-range explorations, but we note that noise2read may yield a slightly different result at different trials, even setting the same seeds.